Chromosomal assignment of canine THADA gene to CFA 10q25

abstract: ...Background Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. THADA (thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs ( Canis lupus familiaris ) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the THADA gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine THADA gene to canine chromosome 10. A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine THADA . Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing. Canine whole blood was incubated with colcemid in order to arrest the cultured cells in metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in fluorescence in situ hybridization (FISH). Ten well spread metaphases were examined indicating a signal on canine chromosome 10 on both chromatids. A detailed fine mapping was performed indicating the canine THADA gene locus on the q-arm of chromosome 10. Results The canine THADA gene locus was mapped on chromosome 10q25. Our mapping results obtained in this study following the previously described nomenclature for the canine karyotype. Conclusion We analysed whether the THADA gene locus is a hotspot of canine chromosomal rearrangements in canine neoplastic lesions of the thyroid and in addition might play a role as a candidate gene for a possible malignant transformation of canine thyroid adenomas. Although the available cytogenetic data of canine thyroid adenomas are still insufficient the chromosomal region to which the canine THADA has been mapped seems to be no hotspot of chromosomal aberrations seen in canine thyroid adenomas.

...Mol Cytogenet Mol Cytogenet Molecular Cytogenetics 1755-8166 BioMed Central 18522714 2430699 1755-8166-1-11 10.1186/1755-8166-1-11 Short Report Chromosomal assignment of canine THADA gene to CFA 10q25 Soller Jan T 1 2 soller@uni-bremen.de Beuing Claudia 2 claudia.beuing@tiho-hannover.de Murua Escobar Hugo 1 2 escobar@uni-bremen.de Winkler Susanne 1 winklers@uni-bremen.de Reimann-Berg Nicola 1 nicola.reimann-berg@uni-bremen.de Drieschner Norbert 1 norbert.drieschner@uni-bremen.de Dolf Gaudenz 3

... ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. THADA (thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs ( Canis lupus familiaris ) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the THADA gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine THADA gene to canine chromosome 10. A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine THADA . Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing. Canine whole blood was incubated with colcemid in order to arrest the cultured cells in metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in fluorescence in situ hybridization (FISH). Ten well spread metaphases were examined indicating a signal on canine chromosome 10 on both chromatids. A detailed fine mapping was performed indicating the canine THADA gene locus on the q-arm of chromosome 10. Results The canine THADA gene locus was mapped on chromosome 10q25. Our mapping results obtained in this study following the previously described nomenclature for the canine karyotype. Conclusion We analysed whether the THADA gene locus is a hotspot of canine chromosomal rearrangements in canine neoplastic lesions of the thyroid and in addition might play a role as a candidate gene for a possible malignant transformation of canine thyroid adenomas. Although the available cytogenetic data of canine thyroid adenomas are still insufficient the chromosomal region to which the canine THADA has been mapped seems to be no hotspot of chromosomal aberrations seen in canine thyroid adenomas. Background In human thyroid adenomas chromosomal translocations involving the regions 19q13 and 2p21 have frequently been described [ 1 , 2 ]. Chromosomal aberrations showing 2p21 rearrangements belong to the most common abnormalities in benign epithelial tumours with an observed frequency of 10% [ 3 ]. Recently, a gene named THADA (thyroid adenoma

... canine thyroid neoplasias [ 11 ]. In order to elucidate whether human genes which are involved in the pathogenesis of benign thyroid adenomas could play a role as orthologous candidate genes for a possible malignant transformation of thyroid tumours in dogs, we have mapped the canine THADA gene and analysed if the canine gene locus is involved in cytogenetic rearrangements. Methods BAC library screening A PCR reaction for PCR-based screening of the Canis familiaris DogBAC library [ 12 ] (Institute of

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Gene: THADA - thyroid adenoma associated - (NCBI)

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