Histological characterization of orphan transporter MCT14 (SLC16A14) shows abundant expression in mouse CNS and kidney

abstract: ...Background MCT14 (SLC16A14) is an orphan member of the monocarboxylate transporter (MCT) family, also known as the SLC16 family of secondary active transmembrane transporters. Available expression data for this transporter is limited, and in this paper we aim to characterize MCT14 with respect to tissue distribution and cellular localization in mouse brain. Results Using qPCR, we found that Slc16a14 mRNA was highly abundant in mouse kidney and moderately in central nervous system, testis, uterus and liver. Using immunohistochemistry and in situ hybridization, we determined that MCT14 was highly expressed in excitatory and inhibitory neurons as well as epithelial cells in the mouse brain. The expression was exclusively localized to the soma of neurons. Furthermore, we showed with our phylogenetic analysis that MCT14 most closely relate to the aromatic amino acid- and thyroid-hormone transporters MCT8 (SLC16A2) and MCT10 (SLC16A10), in addition to the carnitine transporter MCT9 (SLC16A9). Conclusions We provide here the first histological mapping of MCT14 in the brain and our data are consistent with the hypothesis that MCT14 is a neuronal aromatic-amino-acid transporter. Electronic supplementary material The online version of this article (doi:10.1186/s12868-016-0274-7) contains supplementary material, which is available to authorized users.

... MCT8 (SLC16A2) and MCT10 (SLC16A10), in addition to the carnitine transporter MCT9 (SLC16A9). Conclusions We provide here the first histological mapping of MCT14 in the brain and our data are consistent with the hypothesis that MCT14 is a neuronal aromatic-amino-acid transporter. Electronic supplementary material The online version of this article (doi:10.1186/s12868-016-0274-7) contains supplementary material, which is available to authorized users. Keywords Monocarboxylate Transporter Mouse Amino

... also called the monocarboxylate transporters (MCTs). The SLC36 [ 10 ] and SLC5 [ 11 , 12 ] are other SLC-families involved in the transport of monocarboxylates. Only six of the fourteen members of the MCT family have been characterized in detail: SLC16A1, SLC16A7, SLC16A8, SLC16A13, SLC16A2 and SLC16A10, corresponding to MCT14, MCT8 and MCT10 respectively. The inconsistent nomenclature is due to the naming of MCTs in the order in which they were functionally characterized, whereas SLC16-family

... transport. As for MCT14, its substrate profile is still unknown and the expression data are limited. MCT14 has been found in bovine mammary gland, in the gastrointestinal tracts of cattle and the mammary glands of lactating cows [ 21 23 ], but there is no detailed immunohistochemical analysis of MCT14 expression in mouse brain. In this paper we show that Slc16a14 is phylogenetically most closely related to Slc16a9 , Slc16a2 and Slc16a10 . We also mapped the expression of MCT14 throughout the mouse

... and Slc16a10 . Phylogenetic tree showing the evolutionary relationship between the members in the mouse and human monocarboxylate transporter family. MrBayes 3.2.2 [ 25 , 26 ] was used to construct the tree with branch lengths representing evolutionary distance. The scale bar represents 0.1 exchanges per site Tissue preparation Tissue preparation for in situ hybridization (ISH) and immunohistochemistry (IHC) was performed as previously described [ 29 ]. RNA-probe synthesis and in situ

..., followed by the Grubbs test (GraphPad software, version 5.0, La Jolla, CA, USA) to remove outliers in the efficiency calculations before correcting the samples for primer efficiency. The GeNorm protocol [ 31 ] identified five stable housekeeping genes, and their geometric means were used as normalization factors for each tissue. Normalized mRNA expression (SD) was plotted. Results Slc16a14 is evolutionarily most closely related to Slc16a9, Slc16a2 and Slc16a10 Phylogenetic reconstruction of the evolutionary relationship of all mouse and human SLC16 sequences (Fig. 1 ) showed that Slc16a14 is most closely related to Slc16a9 , a carnitine transporter [ 19 , 20 ]. The Slc16a9 and Slc16a14 group form a sister clade to the Slc16a2 and Slc16a10 group, which are transporters for thyroid hormone ( Slc16a2 ) [ 16 , 32 ] and aromatic amino acids as well as iodothyronine ( Slc16a10 ) [ 33 , 34 ]. Identical topology was obtained with maximum likelihood analysis using RAxML [ 35 ] (data not shown). Extensive

... Only 6 of the 14 members of the MCT family have been studied extensively: MCT14, MCT8, and MCT10. The remaining transporters have yet to be characterized in detail. Here we aimed to elucidate the tissue distribution of MCT14, determine its cellular localization, and study its evolutionary relationship to other members of the monocarboxylate transporter family. We performed a phylogenetic analysis revealing a close relationship between Slc16a14 and Slc16a2 , Slc16a9 , and Slc16a10 . QRT-PCR on mouse

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Gene: SLC16A10 - solute carrier family 16 (aromatic amino acid transporter), member 10 - (NCBI)

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